Enriched retinal ganglion cells derived from human embryonic stem cells

KP Gill; SSC Hung; A Sharov; CY Lo; K Needham; GE Lidgerwood; S Jackson; DE Crombie; BA Nayagam; AL Cook; AW Hewitt; A Pébay; RCB Wong

Journal article

Enriched retinal ganglion cells derived from human embryonic stem cells

KP Gill, SSC Hung, A Sharov, CY Lo, K Needham, GE Lidgerwood, S Jackson, DE Crombie, BA Nayagam, AL Cook, AW Hewitt, A Pébay, RCB Wong

Scientific Reports | NATURE PORTFOLIO | Published : 2016

DOI: 10.1038/srep30552

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Abstract

Optic neuropathies are characterised by a loss of retinal ganglion cells (RGCs) that lead to vision impairment. Development of cell therapy requires a better understanding of the signals that direct stem cells into RGCs. Human embryonic stem cells (hESCs) represent an unlimited cellular source for generation of human RGCs in vitro. In this study, we present a 45-day protocol that utilises magnetic activated cell sorting to generate enriched population of RGCs via stepwise retinal differentiation using hESCs. We performed an extensive characterization of these stem cell-derived RGCs by examining the gene and protein expressions of a panel of neural/RGC markers. Furthermore, whole transcriptom..

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University of Melbourne Researchers

Sandy Hung Author

Grace Lidgerwood Author

Bryony Nayagam Author

Alice Pebay Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by grants from the National Health and Medical Research Council (RCBW, 1084256), Bright Focus Foundation (AWH, AP), the University of Melbourne (RCBW, AP), Retina Australia (RCBW, SSCH, AWH, AP) the Ophthalmic Research Institute of Australia (RCBW, SSCH, AWH, AP), National Stem Cell Foundation of Australia (AP), Clifford Craig Medical Research Trust (#133, ALC, AWH, AP), a NHMRC Career Development Fellowship (AP, 1004164), a NHMRC Early Career Fellowship (AWH), a NHMRC-CSL Gustav Nossal postgraduate research scholarship (DEC), an Australian Research Council Future Fellowship (AP, FT140100047), a Cranbourne Foundation Fellowship (RCBW), an Australian Postgraduate Award (KPG), a Garnett Passe and Rodney Williams Memorial Foundation Research Fellowship (BAN), a Senior Wagstaff Fellowship in Otolaryngology (KN), Intramural Research Program of the National Institute on Aging (AS) and operational infrastructure support from the Victorian Government.